产品货号：
GS2135
中文名称：
Bpu10I内切酶
英文名称：
Bpu10I
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration may result in star activity or incomplete DNA cleavage.We recommend increasing incubation time instead of using an excess of Bpu10I.The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.
技术规格
相关搜索：Bpu10I内切酶，Bpu10I限制性内切酶
使用说明
High enzyme concentration may result in star activity or incomplete DNA cleavage.We recommend increasing incubation time instead of using an excess of Bpu10I.The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | CC↑TNAGC |
| 来源 | E.coli strain,that carries recombinant plasmids |
| Assayed on | Lambda DNA |
| Optimal buffer | K(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;100 mM KCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;50%glycerol.Store at -20℃ |
| Ligations | After 5-fold overdigestion with enzyme 80%of the DNA fragments can be ligated.Of these 90%can be recut.In the presence of 10%PEG ligation is better. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 1 hours at 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
相关搜索：Bpu10I内切酶，Bpu10I限制性内切酶